Information such as particle shape, molecular weight, purity, association between molecules can be obtained from the sedimentation pattern of particles in the cell of a rotor rotating at high speed.
(High Performance Liquid Chromatography) The mixture is placed on a stream of liquid called mobile phase and moved in a solid or liquid medium called stationary phase to separate the components. The molecular weight can be calculated by the MS detector.
Fluorescent plate reader
The fluorescence and luminescence intensity of the cell culture plate can be measured.
An analyzer that uses a crystal oscillator (also known as a crystal oscillator). Since it can detect minute masses on the order of nanograms, it is also used for analysis of adsorption phenomena on the surface and detection of biopolymers (proteins, etc.).
A device for slicing frozen tissue that provides samples in microscopic observation of tissue.
A vibrating density hydrometer that can measure liquid samples quickly and with high accuracy.
A device that titrates a ligand solution against a biopolymer solution maintained at an arbitrary temperature within 2 to 80 ° C. and directly measures the heat of reaction of intermolecular interaction caused by mixing the two solutions. By using this device, the coupling constant, the coupling thermodynamic quantity, and the number of coupling sites can be obtained.
Since it can be measured at a higher temperature than high performance liquid chromatography (HPLC), it can be separated at a high pressure.
DLS (2 units)
By capturing the movement of particles in solution, it is possible to measure zeta potential, particle size, and molecular weight of macromolecules as well as parameters for particle dispersion and aggregation.
A device can analyze individual cells by flowing and measure cell suspension at high speed. It is possible to show differences in the relative size, shape, and internal structure of each cell.
Fluorescent and chemiluminescence dyed for gel electrophoresis can be observed.
A device that automatically purifies synthetic compounds.
Using the resonant mass measurement method, it is possible to measure the number of particles, particle size, particle mass, molecular concentration, etc. of protein aggregation in and out of the range of 200 nm to 5 μm.
Qualitative analysis (identification) and quantitative analysis can be performed by irradiating light in the wavelength range of the ultraviolet and visible region at which the sample absorbs. It also can measure the ratio (transmittance) of light transmitted to the opposite side of the incident direction.
GPC-MALS (3 units)
Gel permeation chromatography. A column filled with a porous filler that utilizes a "Size Exclusion" mechanism between the pores on the surface of the filler and the polymer. Not only the average molecular weight can be obtained, but also the "molecular weight distribution" peculiar to the polymer can be obtained. Furthermore, by taking advantages of MALS (multi-angle light scattering detector), it is possible to detect the absolute molecular weight and the radius of inertia.
FFF-MALS (2 units)
Field flow fractionation. A method that uses a method to separate the size of macromolecules and fine particles dissolved or dispersed in a liquid without using columns. Not only the average molecular weight but also the "molecular weight distribution" peculiar to the sample can be obtained. Furthermore, the combination with MALS (multi-angle light scattering detector), it is possible to detect accurately the absolute molecular weight and the radius of inertia.
Ultracentrifugation for purification
By using a high rotation speed, it is possible to separate components that cannot be separated by a normal centrifuge.
Spheryx's unique patented Total Holographic characterization® (THC) method detects individual particles and simultaneously measures the particle size and refractive index of the particles.
UV plate reader
A device measures the absorption at specific wavelength. It is used in biological test such as: cytotoxicity tests, ELISA tests.
When a substance with a chiral internal structure absorbs circularly polarized light, there is a difference in absorbance between left-handed circularly polarized light and right-handed circularly polarized light (circular dichroism). It is possible to obtain useful information on the three-dimensional structure of other optically active substances. It also provides useful information when a substance that is not an optically active substance interacts with a polymer substance and induces chirality.
A device grinds cells from tissues sampled and collects molecules and macromolecules.
Altitude differential refractometer
It measures refractive index (dn / dc) for determining the weight average molecular weight in static light scattering method. The absolute molecular weight can be calculated by using this dn / dc.